Kay Padget
Associate Professor, School of Medicine
Biography
Following completion of a BSc (Hons) in Biomedical science, Dr Padget gained her PhD from the University of Newcastle, UK in 1998 with a thesis entitled: DNA Topoisomerase II in Acute Lymphoblastic Leukemia. Dr Padget continued her research at Newcastle University on elucidating the molecular pharmacology of DNA Topoisomerase II targeting anticancer agents and mechanisms of DNA repair. In 2007 Dr. Padget became a Senior Lecturer in Biochemistry at Northumbria University, UK where she combined her research interests with teaching students from both Northumbria University and St. Georges University International Medical Pathway.
Dr. Padget joined St. George’s University in August 2023 as an Associate Professor in the School of Medicine, Department of Biochemistry. In addition to teaching, Dr. Padget is actively involved in a research collaboration to understand the role of maternal folate in childhood leukemia initiation events which is funded by the World Cancer Research Fund.
Contact Information
Areas of Interest
- In collaboration, Dr Padget is involved in investigating the mechanisms of the developmental origins of translocations seen in childhood leukemia and therapy-related translocations.
Affiliations
- Senior Fellow of Higher Education Academy
- Member of the Biochemical Society
Selected Publications
- Brindle A, Bainbridge C, Kumar MR, Todryk S, Padget K. (2023). The Bisdioxopiperazine ICRF-193 Attenuates LPS-induced IL-1β Secretion by Macrophages. Inflammation (2023). Online ahead of print. https://doi.org/10.1007/s10753-023-01895-2.
- Kritikaki E, Asterling R, Ward L, Padget K, Barreiro E, Simoes DCM. (2021). Exercise Training-Induced Extracellular Matrix Protein Adaptation in Locomotor Muscles: A Systematic Review. Cells. Apr 26;10(5):1022. https://doi.org/10.3390/cells10051022
- Lee KC, Swan R, Sondka Z, Padget K, Cowell I, Austin CA. (2018). Effect of TDP2 on the level of TOP2-DNA complexes and SUMOylated TOP2-DNA complexes. Int J Mol Sci.19(7). pii: E2056. https://doi.org/10.3390/ijms19072056
- Manville C, Smith K, Sondka Z, Rance H, Cockell S, Cowell I, Lee K, Morris N, Padget K, Jackson G, Austin CA. (2015). Genome wide ChIP-seq analysis of human TOP2B occupancy in MCF7 breast cancer epithelial cells. Biology Open 4(11): 1436-1447. https://doi.org/10.1242/bio.014308
- Jaedicke KM, Roythorne A, Padget K, Todryk S, Preshaw PM, Taylor JJ. (2013). Leptin up-regulates TLR2 in human monocytes. J Leukoc Biol. 93(4): 561-71. https://doi.org/10.1189/jlb.1211606
- Lee KC, Padget K, Curtis H, Cowell IG, Moiani D, Sondka Z, Morris NJ, Jackson GH, Cockell SJ, Tainer JA, Austin CA. (2012). MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA. Biol Open. Sep 15;1 (9):863-73. https://doi.org/10.1242/bio.20121834
- Cowell IG, Sondka Z, Smith K, Lee KC, Manville CM, Sidorczuk-Lesthuruge M, Rance HA, Padget K, Jackson GH, Adachi N, Austin CA. (2012). Model for MLL translocations in therapy-related leukemia involving topoisomerase IIβ-mediated DNA strand breaks and gene proximity. Proc Natl Acad Sci U S A. Jun 5;109 (23):8989-94. https://doi.org/10.1073/pnas.1204406109
- Cowell IG, Papageorgiou N, Padget K, Watters GP, Austin CA. (2011). Histone deacetylase inhibition redistributes topoisomerase IIβ from heterochromatin to euchromatin. Nucleus. Jan-Feb;2(1):61-71. https://doi.org/10.1074/jbc.C000824200
- Lopez-Lazaro M, Willmore E, Jobson A, Gilroy K, Curtis H, Padget K, Austin CA. (2007). Curcumin induces topoisomerase I and II – DNA complexes in K562 human leukemia cells. J. Nat. Prod. 70: 1884-1888. https://doi.org/10.1021/np070332i
- Gilroy KL, Leontiou C, Padget K, Lakey JH, Austin CA. (2006). mAMSA resistant human topoisomerase II beta mutation G465D has reduced ATP hydrolysis activity. Nucleic Acids research. 34:1597-1607. https://doi.org/10.1093/nar/gkl057
- Johnson CA, Padget K, Austin CA, Turner BM. (2001). Deacetylase activity associates with topoisomerase II and is necessary for etoposide induced apoptosis. J. Biol. Chem. 276: 4539-4542. https://doi.org/10.1074/jbc.C000824200
- Padget K, Pearson ADJ, Austin CA. (2000). Quantitation of DNA topoisomerase II alpha and beta in human leukemia cells by immunoblotting. Leukemia 14: 1997-2005. https://doi.org/10.1038/sj.leu.2401928
- Padget K, Stewart A, Charlton P, Tilby MJ, Austin CA. (2000). An investigation into the formation of N-[2-(dimethylamino)ethyl]acridine-4-carboxamide (DACA) and 6-[2-(dimethylamino)ethylamino]-3-hydroxy-7H-indeno[2,1-C]quinolin-7-one dihydrochloride (TAS-103) stabilized DNA topoisomerase I and II cleavable complexes in human leukemia cells. Biochemical Pharmacology. 60:817-821. https://doi.org/10.1016/S0006-2952(00)00402-0
- Padget K, Carr R, Pearson ADJ, Tilby MJ, Austin CA. (2000). Camptothecin stabilized topoisomerase I-DNA complexes in leukemia cells visualized and quantified in situ by the TARDIS assay. Biochemical Pharmacology. 59(6):629-638. https://doi.org/10.1016/S0006-2952(99)00372-X
- Cowell IG, Okorokov, AL, Cutts SA, Padget K, Bell M, Milner J, Austin CA. (2000). Human topoisomerase II alpha and II beta interact with the C-terminal region of p53. Exp Cell Res. 255(1):86-94. https://doi.org/10.1006/excr.1999.4772
- Willmore E, Frank AJ, Padget K, Tilby MJ, Austin CA. (1998). Etoposide targets topoisomerase II alpha and II beta in leukemic cells: isoform specific cleavable complexes visualized and quantified in situ by a novel immunofluorescence technique. Molecular Pharmacology 53:78-85. https://doi.org/10.1124/mol.54.1.78
Selected Projects
- The role of maternal folate in childhood leukemia initiation: understanding the molecular mechanism. Co-applicant with Prof. J. McKay. Funded by the World Cancer Research Fund, Nov. 2022 – Nov. 2025.
- Environmental causes of childhood leukemia-associated chromosomal translocations. Co-applicant with Prof J McKay. Funded by Children with Cancer UK. Jan 2020 – Oct 2022.
- The Role of Topoisomerase in Neuronal Aging and DNA repair. Northumbria University Funded PhD Studentship. Sept. 2016 – Sept. 2019.
- The Role of Chromatin Dynamics and Topoisomerase II and the Immune Response. Northumbria University Funded PhD Studentship. Jan. 2011- Jan. 2014.
- Molecular Pharmacology of Topoisomerase II and its Potential Role in Generating the Chromosomal Translocations that cause Therapy Related Leukemia. Joint with Prof. C.A. Austin and Dr. G.H. Jackson. Funded by the Leukemia Research Fund. Jan. 2008- Jan. 2013.
- Potentiation of Etoposide by HDAC Inhibitors in Small Cell Lung Cancers and Non- Small Cell Lung Cancers. Joint with Prof. C.A. Austin. Funded by the Association for International Cancer Research Apr. 2005 - Dec. 2009.
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